In wild-type 4-cell stage embryos, the APH-2 protein is associated with the surface membranes of all four cells. Presenilin-deficient embryos (
sel-12(
ty11);
hop-1(RNAi) showed an abnormal accumulation of APH-2 around the nucleus in an apparently identical pattern to that of
aph-1 mutant embryos. (see Gene_regulation
cgc5082_aph-2.a).